E3222-75D

Enolase, Yeast (2-Phospho-D-glycerate hydrolyase, EC4.2.1.11)

Size

£451.00

SKU:: E3222-75D
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as solution in phosphate buffer, pH 7.2, 50% glycerol.
Extra Details:: Enolase, also known as phosphopyruvate hydratase, is a metalloenzyme responsible for the catalysis of the conversion of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP), the ninth and penultimate step of glycolysis. Enolase belongs to the class Lyase. Enolase can also catalyze the reverse reaction, depending on environmental concentrations of substrates.[3] The optimum pH for this enzyme is 6.5. Enolase is present in all tissues and organisms capable of glycolysis or fermentation. The enzyme was discovered by Lohmann and Meyerhof in 1934 and has since been isolated from a variety of sources including yeast, human muscle and erythrocytes. Standard: Enolase-05 Contaminants: (as Enolase activity = 100%) Phosphoglyceromutase: ≤0.02% Pyruvate kinase: ≤0.02% Specific Activity: ≥40u/mg protein (at 25°C and pH7.5) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.