E3220-09

Enkephalin Leu5

Size

£704.00

SKU:: E3220-09
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Leu5-enkephalin, conjugated to BSA
Clone:: 2Q51 (NOC1)
Format:: Supplied as a liquid in PBS, <0.1% thimerosal.
Extra Details:: Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level. Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:100-1:400. Cryostat sections of rat and guinea pig brain stems. Immunocytochemistry: 1:300. Primary cultures of rat striatal and hippocampal neurons using Cy2 or Cy3 conjugated secondary antibody for detection. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.