E2291-40A

Endonuclease G (EndoG)

Size

£722.00

SKU:: E2291-40A
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa55-70, GGPRGPGELAKYGLP, from human EndoG. Species Sequence Homology: mouse and rat
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml, detects a band of ~35kD in HepG2 and 3T3 cell lysates. Immunohistochemistry (paraffin): 15ug/ml. Requires antigen retrieval using heat treatment prior to staining. sodium citrate buffer pH 6.0 is recommended for this purpose. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Human pancreas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.