Emmprin (Extracellular Matrix Metalloproteinase (MMP) Inducer) (MaxLight 650)

Size

£1,042.00

SKU:: E2260-03-ML650
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the N-terminal region of the human Emmprin protein.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Extra Details:: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Degradation of the basement membrane by matrix metalloproteinases (MMPs) is an important step in the growth, invasion, metastasis, and angiogenesis of human tumors. MMP-2 and MMP-9 are key enzymes in the degradation of Type IV collagens, a major component of the basement membrane in cancer tissues. MMPs are secreted by stromal fibroblasts near tumor cell clusters, and may also be secreted by tumor cells themselves. EMMPRIN, or extracellular matrix metalloproteinase inducer, is a 58kD protein with two immunoglobulin domains that was originally purified from the plasma membrane of the human LX-1 lung carcinoma cell line. EMMPRIN has been localized on the outer surface of tumor cells, where it interacts with fibroblasts to stimulate the expression of MMPs. Although the mechanism of EMMPRIN’s regulatory action on MMPs is unknown, the p38 MAP kinase pathway has been implicated in the induction of MMP in dermal fibroblasts. Applications: Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: FLISA: 0.1-1.0ug/ml Western Blot: 1-5ug/ml Immunohistochemistry (FFPE): 10ug/ml Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to IHC staining. Optimal dilutions to be determined by the researcher. Positive Control: Human breast cancer BT-20 and colon cancer SW480 cell lines. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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