E2250-05A

Elongin B, Recombinant Human (Transcription elongation factor B polypeptide 2, TCEB2, RNA polymerase II transcription factor SIII subunit B, SIII p18, EloB, Elongin 18kD subunit)

Size

£422.00

SKU:: E2250-05A
Molecular Weight:: 13.1
Purity:: ≥95% by SDS-PAGE
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mm Tris-HCl buffer, pH 8.0, 1mMDTT, 0.1mm PMSF, 20% glycerol.
Extra Details:: Elongin B , also known as TCEB2, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimer consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant TCEB2 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Source: Human, Recombinant, E. coli, Elongin B,1-118aa AA Sequence: MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.