Elastase, Pancreatic, Rat

Size

£698.00

SKU:: E2236-23
Molecular Weight:: 25.7
Purity:: ~95% by SDS-PAGE
Storage Conditions:: 4°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Salt free. Reconstitute with 0.05M sodium acetate, 0.2M sodium chloride, pH 5.0.
Extra Details:: Elastase, like trypsin and chymotrypsin, is a serine protease that also hydrolyses amides and esters. It is produced in the pancreas as an inactive zymogen, proelastase, and activated in the duodenum by trypsin. Elastase is distinctive in that it acts upon elastin. Because elastin in found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. Contaminants: Does not contain trypsin or chymotrypsin. Specific Activity: ~2.0U/mg. One unit will hydrolyze 1umole of Suc-(Al)3-pNA per minute at pH 8.8 and 37°C. Extinction Coefficient: 0.985 Activity Assay: 5-10ug in 0.1M Tris, pH 8.8 at 37ºC containing 0.2mM Suc-[Al]3-pNA Storage and Stability: Lyophilized powder may be stored at 4°C. Stable for 12 months at 4°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.