Elastase, Leucocyte Human (ELANE, ELA2, Neutrophil elastase, Elastase-2, Human leukocyte elastase) (Not for Export EU)

Size

£607.00

SKU:: E2236-21C
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human neutrophil elastase
Format:: Supplied as a lyophilized powder. Reconstitute with sterile 250ul sterile ddH2O.
Extra Details:: Applications: Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 -1:2000 Western Blot: 1:200 -1:500 Immunohistochemistry: 1:50 -1:100 Immunocytochemistry: 1:50 -1:100 Optimal dilutions to be determined by the researcher. Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.