E2212-87
eEF1A (Eukaryotic Translation Elongation Factor 1 alpha 1)
Size
£581.00
- SKU:
- E2212-87
- Purity:
- Purified by peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A. Species Sequence Homology: Xenopus.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A variety of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells. eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and then interacts with amino acyl-tRNAs and promotes the recruitment of amino acyl-tRNAs to the A-site of the ribosome. After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B. Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IF-IC): 1:25 Western Blot: 1:1000; Band at ~50kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice