E2212-55
Effector Cell Protease Receptor-1 (EPR-1)
Size
£754.00
- SKU:
- E2212-55
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 20aa from human EPR -1. Peptide has no significant homology with survivin.
- Format:
- Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.09% sodium azide, 40% glycerol.
- Extra Details:
- The inhibitors of apoptosis proteins (IAPs) are a widely expressed gene family of apoptotic inhibitors. Recently, a new human gene encoding a structurally and unique IAP designated Survivin has been identified. Survivin (human 142 aa, ~16.5kD) is highly expressed in less-differentiated embryonic cells or rapidly dividing tumor cell but not in fully differentiated adult tissues. Interestingly, Survivin coding strand has significant sequence homology with Effector cell Protease Receptor-1 (EPR-1) suggesting a potential for functional interaction between these two proteins. Factor Xa plays a critical role in the coagulation process by catalyzing the activation of prothrombin to thrombin. It acts on leukocytes, endothelium and smooth muscle cells triggering complex pathways of intracellular signaling. Factor Xa binds to EPR-1. The full length EPR-1 predicts a protein of 337 aa (~65kD de-glycosylated form). EPR-1 is expressed in vascular endothelial cells and smooth muscle cells. Survivin and EPR-1 are encoded by structurally and topographically distinct messages from potentially originating from gene cluster at chromosome 17q25. Overexpression of EPR-1 increased apoptosis and inhibited growth of transformed cells. The importance of the Survivin-EPR-1 interaction remains to be elucidated. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. Detects a ~62kD protein in detergent solubilized extracts of Jurkat and monocytic THP-1 cells. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice