E2208
EDG 1 (EDG-1, Endothelial Cell Differentiation Gene 1, Sphingosine 1 Phosphate Receptor 1)
Size
£832.00
- SKU:
- E2208
- Application:
- WB
- Purity:
- Purified by ammonium sulfate precipitation.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the C-terminal region of the human EDG-1 receptor.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.08% sodium azide.
- Extra Details:
- EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte- containing and other tissues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 5-10ug/ml, detects an ~44kD band in RH7777 cells transfected with full length human EDG1. Optimal dilutions to be determined by the researcher. Positive Control: RH7777 cells transfected with full length EDG-1 protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice