EDG 3 (Endothelial Cell Differentiation Gene 63, Sphingosine-1-phosphate Receptor 3, S1PR3)

Size

£616.00

SKU:: E2208-15B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa12-25, VRGNETLREHYQYV of human EDG 3 protein
Format:: Supplied as a liquid in TBS, pH 7.4, 5mg/ml BSA.
Extra Details:: Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8. S1P3 couples to Gi/o-ERK, Gq-PLC, and G12/13-Rho axes to mediate S1P-induced cell proliferation, survival, migration, and related signaling events. S1P3 is widely expressed in various tissues, suggesting diverse physiological functions of this receptor. The human and murine S1P3 have 378 amino acids with an estimated molecular weight of 42kD. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at different positions in SDS-PAGE. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:200 Immunocytochemistry: 1;100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.