E2208-01D
EDG1 (Endothelial Cell Differentiation Gene 1) (APC)
Size
£863.00
- SKU:
- E2208-01D
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma tissue culture supernatant.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Ser382 from human EDG1 expressed in NS0 cells.
- Clone:
- 8H120
- Format:
- Supplied as a liquid in saline, ≤0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG-1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 human EDG1/S1P1-transfected CHO-S cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, 10ul of E2208-01D was added to up to 1x10e6 cells and incubated for 30 minutes at RT. 3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. The cells were resuspended in F4568-10 for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2b antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice