EBF1 (COE1, Early B Cell factor 1, COE1, EBF, O/E-1, OLF1, Collier, Olf and EBF transcription factor 1, early B Cell factor olfactory neuronal transcription factor 1)

Size

£642.00

SKU:: E0999-05
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-HNQLPALANTSV, from the internal region of human EBF1 (NP_076870.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:64,000 Western Blot: 0.3-0.1ug/ml detects a band at ~75kD observed in human Adipose lysates (calculatd MW: 64.5kD according to NP_076870.1) Immunoprecipitation: reported to immunoprecipitate EBF1 from induced 3T3-L1 expressing FLAG-EBF1 Optimal dilutions to be determined by the researcher. Positive Control: Human Adipose lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.