E0255-05A

EAT-4, Rat (Vesicular Type Transporters for Glutamate, VGLUT) Control Peptide

Size

£416.00

SKU:: E0255-05A
Purity:: Highly purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.2
Extra Details:: An 18aa synthetic peptide witin the N-terminus of C. elegans EAT-4 was synthesized, conjugated to KLH, and antibodies generated in rabbits. Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Most recently, vesicular type transporters for glutamate, termed VGLUTs (VGLUT1/BNPI, VGLUT2/DNPI, and EAT-4), have been cloned and characterized that are related to phosphate transporters. Although neurons exhibit Na-dependent Pi transport, the biological role of Pi uptake is not clear. Proximal tubules in the kidney reabsorb Pi in the glomeruli by the action of a group of phosphate transporters (Type1-NaPi related, type2-NaPi-2 related, and type 3-viral receptor-related. These receptors show weak (~20% identity) between various subtypes. A distinct type of brain specific Na+-dependent phosphate (Pi) transporter (BNPI), originally characterized as a plasma membrane transporter has been localized in a subset of glutamatergic neurons (amygdla,cereberal cortex, and hippocmaplus) and identified as VGLUT1 (rat and human 560 aa; ~60kD, ~30% homology with type-1 Pi-transporters). VGLUT1 shows strong sequence homology to EAT-4, a C. elegans protein that appears to have specific presynaptioc role in glutamtergic transmission. Loss of function mutations in EAT-4 affects multiple glutamregic neurotransmission pathways. EAT4 encodes a protein of 563 aa (~48% identity with BNPI and ~30% identity with known NaPi-transporters found in mammalian kidney). Like BNPI, EAT-4 is also expressed in glutamatergic neurons.

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