E0014

E2F4 Transcription Factor

Size

£800.00

SKU:: E0014
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fusion Protein corresponding to the human E2F-4 protein.
Clone:: 0.T.33 (GG22-2A6)
Format:: Supplied as liquid in 0.1M Tris-glycine buffer, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Extra Details:: E2f transcription factors are functionally regulated by binding to RB p110, p107, and p130. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detected E2F4 in RIPA lysates from non-stimulated A431 cell lysate. Previous lots have detected E2F4 in HeLa nuclear extract, mouse 3T3 fibroblasts, and rat L6 skeletal myoblasts. Immunoprecipitation: 4-10ug immunoprecipitated E2F4 from 500ug of mouse 3T3/A431 cell lysate Optimal dilutions to be determined by the researcher. Postive Control: A0001-50 included. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.