E0010-20-BIOTIN
E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3) (Biotin)
Size
£1,049.00
- SKU:
- E0010-20-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human E2F-1 protein.
- Clone:
- KH95
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Extra Details:
- E2F-1 is a transcription factor (437 amino acids, 46kD) that contains a cyclin A binding domain, a DNA binding domain, a dimerization domain, and a transactivation domain. E2F-1 is expressed in a wide variety of cell lines and tissues and is expressed broadly, but not uniformly, during mouse embryogenesis. E2F-1 binds almost exclusively to pRB and phosphorylation of E2F-1 may disrupt pRB/E2F complexes. Overexpression of E2F-1 or dE2F in normal cells causes high levels of apoptosis while p19 arf -deficient cells are partially resistant to E2F-1-induced apoptosis. p53-independent apoptosis requires the E2F-1 DNA-binding domain but not an intact transactivation domain. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice