E-Tag (GAPVPYPDPLEPR)

Size

£448.00

SKU:: E0004-01E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues GAPVPYPDPLEPR coupled to KLH.
Format:: Supplied as a lyophilized powder. Reconstitute with 1ml sterile PBS, pH 7.4. No preservative added.
Extra Details:: The E-Tag is a popular epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized E-tag epitope represents the peptide sequence GAPVPYPDPLEPR. Applications: Suitable for use in ELISA, Western Blot and Immunostaining. Other applications not tested. Recommended Dilutions: ELISA: 1:500-1:1000 Western Blot: 1:250-1:500 Immunostaining: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.