D9910-30
Dystrophin, Rod Domain
Size
£825.00
- SKU:
- D9910-30
- Purity:
- Supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bacterial fusion protein
- Clone:
- 2Q1101 (6D3)
- Format:
- Supplied as a liquid in PBS, 1% BSA, 15mM sodium azide.
- Extra Details:
- Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation. Applications: Suitable for use in Western Blot, Immunohistochemistry and EM Gold. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:250 Immunohistochemistry (Fresh frozen, unfixed tissue): Neat 1:20 Not recommended for use on paraffin embedded tissue. EM Gold: Undiluted 90 minute incubation at 25°C. Light fixation with 2% formaldehyde + 0.001% glutaraldehyde for 1 hour. Use 2.3M sucrose as cryoprotectant. Optimal dilutions to be determined by the researcher. Expected Staining Pattern: Light microscopy: Continuous rim of labeling at the periphery of muscle fibers Western Blot: Strong double bands at ~400kD and metabolites of lower molecular mass E.M. Gold: Close to the cytoplasmic face of the plasma membrane. Positive Control: Snap frozen normal human or rat striated muscle. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice