D9901-50
Dynein, Cytoplasmic, 74kD Intermediate Chains
Size
£754.00
- SKU:
- D9901-50
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine brain cytoplasmic dynein
- Clone:
- 2Q46 (74.1)
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
- Extra Details:
- Applications: Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Western Blot: 1:1000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Immunoprecipitation: 10ul/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice