D9845-17A
11-dUTP (Biotin) (5mM)
Size
£2,551.00
- SKU:
- D9845-17A
- Molecular Weight:
- 862.67
- Purity:
- ≥ 95% (by HPLC)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a sterile liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin.
- Extra Details:
- Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications. Applications: Suitable for use in DNA labeling by PCR with taq polymerase, DNA labeling by nick translation with DNase I, DNA labeling by primer extention with Klenow 3’-5’ exo-, 3’ end DNA labeling with terminal deoxynucleotidyl transferase (TdT), cDNA labeling by reverse transcription with Moloney Murine Leukemia Virus Reverse Transcriptase. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Spectroscopic Properties: Wavelength max: 240/289nm Extinction coefficient: 10.7/7.11 mmol-1 cm-1 (Tris-HCl, pH 7.5) Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice