D9845-15

4-dUTP (Biotin) (N-Biotin-5-(3-aminoallyl)-2’-deoxy-uridine-5’-triphosphate, Triethylammonium Salt)

Size

£549.00

SKU:: D9845-15
Molecular Weight:: 749.51
Purity:: ~95%
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin.
Extra Details:: Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications. Applications: Suitable for use in SNP Analsis, In situ hybridization detection, Excision repair patches, Southern Blot and DNA-labeling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Spectroscopic Properties: lambda max 240/289nm; epsilon 10.7/7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.