D9635-51
DSCAM (Down Syndrome Cell Adhesion Molecule)
Size
£812.00
- SKU:
- D9635-51
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Glu18-Met1595 of human DSCAM long isoform expressed in NS0 cells.
- Clone:
- 7H210
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- DSCAM (Down syndrome cell adhesion molecule) is a 230 kD axonal guidance molecule that is a member of the Ig superfamily. It is synthesized as a mature, 2005 amino acid (aa) type I transmembrane glycoprotein. There is a large, 1578 aa extracellular domain (ECD) and a 389 aa cytoplasmic region. The ECD shows ten C2-type Ig-like domains and six fibronectin type III repeats. One soluble splice form is known which contains all the ECD modules and ends after aa # 1571 of the precursor. This form has a unique C-terminal ten amino acids. DSCAM is found on axonal and dendritic processes. It also forms homophilic complexes among cells. Overexpression is associated with Down’s syndrome. The ECD of human is 98% and 77% aa identical to mouse and zebrafish DSCAM ECD, respectively. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml in immersion fixed A172 human glioblastoma cell line Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice