D9452-02

Drebrin (D0S117E, Drebrin 1, DBN 1, DBN1, Developmentally Regulated Brain Protein, DKFZp434D064, Drebrin E)

Size

£856.00

SKU:: D9452-02
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa632-649 from the C-terminal, of Derbrin conjugated to KLH.
Clone:: 5F271
Format:: Supplied as a liquid, 0.09% sodium azide.
Extra Details:: Drebrin is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining is found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Hepatocytes and cross-striated muscle consistently stain negative. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen tissue only): 1:100 with PBS, pH 7.4; Incubation time 1 hr at RT. Optimal dilutions to be determined by the researcher. Recommended Positive Control: Primary human keratinocytes, U333, MCF-7, SV80; B16 (mouse melanoma cells) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice