D9370
DRAM, CT (Damage-Regulated Autophagy Modulator)
Size
£655.00
- SKU:
- D9370
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 16aa from near the C-terminal of human DRAM. Epitope: within aa170-220
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy (1), a process that degrades cytosolic proteins and organelles (reviewed in 2). It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers (3) highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. See D9370-01 for control peptide. Immunohistochemistry: 2.5ug/ml Immunohistochemistry: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: 293 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice