D9334-02B
DRAK1 (DAP Kinase-related Apoptosis-inducing Protein Kinase 1, Death-associated Protein Kinase-related 1, Serine/threonine Kinase 17a Death Associated Protein Kinase Related 1, STK17A)
Size
£800.00
- SKU:
- D9334-02B
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala115-Glu313 from human DRAK1 expressed in E. coli.
- Clone:
- 12K83
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- DRAK1 (DAP kinase-related apoptosis-inducing protein kinase 1; also STK17A is a 55kD member of the DAP kinase subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in osteoclasts, resides in the nucleus, and initiates apoptosis. Human DRAK1 is 414aa in length. It contains an N-terminal polyPro segment aa33-37 followed by a Ser/Thr kinase domain aa64-321 with a catalytic loop aa84-193. Apoptosis apparently requires an intact kinase domain. No rodent DRAK1 has been reported. Over aa115-313, human DRAK1 shares 95% aa identity with canine DRAK1, and 65%aa identity with human DRAK2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using MOLT4 human acute lymphoblastic leukemia cell line and A431 human epithelial carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice