D9300-33A
DR4 (TNFRSF10A, TRAIL R1, TRAIL Receptor1, Death Receptor4)
Size
£594.00
- SKU:
- D9300-33A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa2-21 of human DR4.
- Clone:
- 32A242
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Extra Details:
- Cells undergo programmed cell death (Apoptosis) in response to various stimuli. Apoptosis is essential for morphogenesis, tissue homeostasis, and host defense(1). Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4)(2). The ligand for this novel death receptor has been identified and termed TRAIL(3,4), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1)(5). DR4 is expressed in most human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells. Applications: Suitable for use in Western Blot and Immunocytochemistry/immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml, in HeLa a ~57kD band should be detected. Optimal dilutions to be determined by the researcher. Positive Control: HeLa Whole Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice