D9300-31

DR4, Recombinant, Human (TRAIL R1, TRAIL Receptor1, Death Receptor4)

Size

£332.00

SKU:: D9300-31
Molecular Weight:: 22.7
Purity:: ≥98% by SDS-PAGE and HPLC analyses. Endotoxin: ≤ 0.1ng/ug (1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. No additives. Reconsitute in sterile ddH2O to a concentration of 0.1-1mg/ml, which can then be diluted into other aqueous solutions. Do not vortex.
Extra Details:: TRAIL Receptor-1/DR4 and TRAIL Receptor-2/DR5 belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain, " which can activate the cell's apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L. Recombinant human soluble TRAIL Receptor-1/DR4 is a 22.7 kDa protein (215 amino acid residues) consisting of the TNFR homologous, cysteine rich portion of the extracellular domain. Biological Activity: The specific activity as determined by its inhibitory effect of IL-8 production in human PBMC (peripheral blood mononuclear cells) by human sTRAIL/Apo2L. 95% inhibition (100ng/ml of sTRAIL/Apo2L) was reached using a concentration of 100ng/ml of sTRAIL Receptor-1. Amino Acid Sequence: MSGTGAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASQQLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Ambient