D9150-78B

DPM1 (Dolichol-phosphate Mannosyltransferase, Dolichol-phosphate Mannose Synthase, DPM Synthase, Dolichyl-phosphate beta-D-mannosyltransferase, Mannose-P-dolichol Synthase, MPD Synthase)

Size

£642.00

SKU:: D9150-78B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to RELEVRSPRQNKYS-C, from human DPM1, at N-terminal (NP_003850.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Applications: Suitable for use in Immunohistochemistry, Peptide ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, observed in human liver lysates on ~28kD bands Immunohistochemistry: 3.75ug/ml on paraffin embedded human tonsil Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.