Dopamine Receptor, D3

Size

£891.00

SKU:: D9017
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide 19aa sequence from the rat D3 receptor within the 3rd cytoplasmic domain. Species Sequence Homology: Mouse: 100%; human/monkey: 63%.
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: This gene encodes the D3 subtype of the dopamine receptor. The D3 subtype inhibits adenylyl cyclase through inhibitory G-proteins. This receptor is expressed in phylogenetically older regions of the brain, suggesting that this receptor plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). Rat Dopamine D3 receptor is a 446aa, G-protein coupled and transmembrane receptor protein (1-2). Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-10ug/ml (ECL). Load ~100-400ug of membrane protein/lane to visualize the receptor band. ELISA: 0.5-1ug/ml (1ug/ml immunogen peptide/well). Immunohistochemistry: 2-10ug/mL. Suggested fixative is 4% PFA, fixation 10-15', permeabilized with 0.3-0.5% triton X-100 for 10-15' (tissue, less time for cells), primary antibody dilution is 1:100 for direct fluorescence Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice