D9017-25
Dopamine Receptor, D3, D3R
Size
£767.00
- SKU:
- D9017-25
- Purity:
- Purified over the peptide-Sepharose column
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 19 AA Peptide (Control peptide) sequence from the rat D3 receptor within the 3rd cytoplasmic domain. The peptide was coupled to KLH
- Format:
- Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.09% sodium azide, 40% glycerol.
- Extra Details:
- Lyophilized products should be reconstituted in 100 ul water and gently mixed for 15 min at room temp. All peptide/antibody received in solution or reconstituted from lyophilized vials should be stored frozen at -20°C or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw. Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. Approx. 100-400ug membrane protein/lane should be loaded to visualize the receptor band. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Immuoprecipitation: Not tested. We suggest 5-10 ul neat serum or 1-10ug affinity pure per 100-400ug of protein samples. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice