D9016-45

Dopamine Receptor, D2S, L, Human, Control Peptide

Size

£384.00

SKU:: D9016-45
Purity:: Highly purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
Extra Details:: Control Peptide for D9016-43 (antiserum) and D9016-44 (affinity purified). Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446aa, G-protein coupled, and transmembrane receptor protein. D2R may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. Source: A 28aa synthetic peptide sequence from the human D2 Receptor, within the cytoplasmic loop # 3, coupled to KLH. The 28aa sequence is shared by both the long and short form. Sequence Homology: Human, bovine, canine-100%; Xenopus-50% with D2 receptors. No significant sequence homology is seen with other dopamine receptors (D1 and D3-D5). Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: Coat plates with 1ug/ml. Antibody Blocking: Use 5-10ug per 1ug of affinity purified antibody (D9016-44) or 1ul of antiserum (D9016-45). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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