Dopamine Receptor, D2L, D2S

Size

£887.00

SKU:: D9016-10
Purity:: Purified by immunoaffinity chromotography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the cytoplasmic loop #3 (28aa) of the human D2 receptor.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Two isoforms are reported for the D2 Dopamine Receptor, isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: 0.5-1.0ug/ml Western Blot: 1:1000. Detected Dopamine Receptor D2 on 10ug of human brain lysates. Immunoprecipitation: 1-10ug/ml. Use 100-400ug of protein. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms. Immunohistochemistry (Paraformaldehyde fixed sections): 2-10ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: Monkey brain tissue, rat brain lysate Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice