D9014-01
Dopamine Receptor, D1a, aa403-415
Size
£891.00
- SKU:
- D9014-01
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 13 amino acid peptide sequence (aa403-415) from rat D1a (1). This peptide is predicted to be cytoplasmic. Species Sequence Homology: porcine or bovine (100%), human (78%) and mouse (92%).
- Format:
- Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide.
- Extra Details:
- This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. The rat D1a receptor is a 446 amino acid, G-protein coupled and transmembrane receptor protein Applications: Suitable for use in Western blot, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1-10ug/ml (Chemiluminescence technique). Approximately 100-400ug of protein/lane should be loaded to visualize the 75kD band Immunohistochemistry: 2-10ug/ml on paraformaldehyde fixed sections. ELISA: 0.5-1ug/ml (1ug/ml D1A peptide/well) Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice