Dopamine Receptor D2 (DRD2)

Size

£486.00

SKU:: D9000-06L
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Dopamine Receptor D2 (Cys271-272-282) covalently attached onto a carrier protein.
Format:: Supplied as a lyophilized powder. Reconstitute with 5ml PBS, 10mg/ml BSA.
Extra Details:: The D2 dopamine receptor is one of five (D1 to D5) receptors for dopamine. Its activity is mediated by G proteins which inhibit adenylyl cyclase. It interacts with GPRASP1, neurabin-2, CLIC6, CADPS and CADPS2. It is a transmembrane protein that belongs to the G-protein coupled receptor 1 family. The unprocessed precursor has a length of 443 amino acids and an estimated molecular mass of 50.6kD. Genetic variations in DRD2 may determine the genetic susceptibility to alcoholism. A missense mutation in this gene causes myoclonus dystonia and several other defects in this gene are thought to be involved in psychiatric disorders, especially in schizophrenia. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Dilute ≥1:8 in 1% normal goat serum, TBS/Tween-20 before use. Optimal dilutions to be determined by the researcher. Included Control Peptide: D9000-06L1: Dopamine Receptor D2 Control Peptide (DRD2) Reconstitute with 600ul of reconstituted antibody. Peptide Amino Acid Sequence (Cys271-272-282) : NH2-Cys-Ala-Ala-Arg-Arg-Ala-Gln-Glu-Leu-Glu-Met-Glu-COOH Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice