Dopamine Receptor D1 (DRD1)

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£607.00

SKU:: D9000-04F
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Dopamine Receptor D1 (Ac-9-21-Cys22) covalently attached onto a carrier protein.
Format:: Supplied as a liquid in PBS. Dilute further in PBS, 10mg/ml BSA.
Extra Details:: Dopamine Receptor D1 (DRD1) belongs to the G-protein coupled receptor 1 family and it is one of the five types of dopamine receptors (D1-D5). DRD1 is the most expressed dopamine receptor in central nervous system and it is involved in the regulation of neuronal growth and development, and modulation of dopamine receptor D2-mediated events. G protein coupled DRD1 activates adenylyl cyclase which leads to activation of cyclic AMP dependent protein kinases. Polymorphism of DRD1 gene has been implicated in bipolar I disorder and attention deficit hyperactivity disorder (ADHD). Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 40ug/ml detected DRD1 at ~50kD using whole rat brain homogenate. Immunocytochemistry: 5ug/ml stains cells in various regions of PLP fixed rat brain sections. These regions include the Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantis Nigra reticulata and the Ventral tegmental area. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice