DOCK2 (Dedicator of Cytokinesis Protein 2, KIAA0209)

Size

£837.00

SKU:: D8080-05M
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Gly420-Gly642 from human DOCK2, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: DOCK2 (Dedicator of cytokinesis protein 2) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Its predicted molecular weight is 210kD, although it may run approximately at 190kD in SDS-PAGE. It is found in hematopoietic cell types, and regulates the activity of the GTPases Rac-1 and -2. Human DOCK2 is 1830aa in length. It contains one SH3 domain (aa8-69), a DHR-1 region that localizes DOCK2 to the plasma membrane (aa420-662), and a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa1115-1621). There are two isoform variants, one that shows a 38aa substitution for Tyr1405, and a second that shows a start site at Met450 with accompanying deletions of aa462-494 and 630-766, and a 22aa substitution for aa934-1830. Over aa420-642, human DOCK2 shares 96% aa identity to mouse DOCK2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice