D8076
DnaK
Size
£508.00
- SKU:
- D8076
- Purity:
- Purified by Protein G affinity chromatography from ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E. coli DnaK
- Clone:
- 4E66
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- DnaK possesses an autophosphorylation activity and a weak 5’-nucleotidase activity, cleaving the 5’-phosphate groups from both ribose- and deoxyribose nucleotides. Genetic and biochemical studies have demonstrated protein-protein interactions between the DnaK protein and the lambdaO and lambdaP DNA replicating proteins. DnaK has also been shown to interact with DnaJ and GrpE heat shock proteins. In addition, DnaK has been demonstrated to preferentially bind to the unfolded form of bovine pancreatic trypsin inhibitor as opposed to the native protein. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology. Like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes, essentially as first proposed by Hightower and Pelham. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:10,000. Detects a band at ~70kD. Optimal dilutions to be determined by researcher. Positive Control: D4015-11: DnaK, Recombinant, E. coli Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice