D8075-83E
DMPK (Myotonin-protein Kinase, MT-PK, DM-kinase, DMK, DM1 Protein Kinase, DMPK, Myotonic Dystrophy Protein Kinase, DM1PK, MDPK)
Size
£825.00
- SKU:
- D8075-83E
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Glu393-Thr543 from human DMPK expressed in E. coli.
- Clone:
- 12K82
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- DMPK also called DMK, MDPK or MT-PK is an 80kD cytoplasmic protein of the ser/thr kinase superfamily (EC 2.7.11.1). The 639aa human DMPK includes a protein kinase domain aa81-349, an AGC kinase C-terminal domain aa350-425 and a coiled-coil negative regulatory domain that mediates dimerization aa467-546. Within the region used as an immunogen, human DMPK shares 72% and 73% aa sequence identity with mouse and rat DMPK, respectively. This region is included within 12 isoforms that range in size from aa530-634. The most prominent, muscle- specific isoform phosphorylates phospholamban and is critical for cardiac contractility. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml in lysates of human heart tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice