D8074-90D
DMC (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1) (Biotin)
Size
£940.00
- SKU:
- D8074-90D
- Application:
- WB
- Purity:
- Purified by mouse DMC affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant mouse rmDMC.
- Format:
- Supplied as a lyophilized powder from PBS with BSA. Reconstitute with 100ul sterile TBS, pH 7.3, 0.1% BSA. Labeled with Biotin.
- Extra Details:
- Dendritic cell- and Monocyte-attracting Chemokine-like protein (DMC), also known as VEGF-induced cytokine (VCC-1), is a secreted molecule that is closely related to CXCL8/IL-8 and CXCL14/BRAK. DMC is constitutively produced by airway and intestinal epithelium and induces the chemotaxis of monocytes and dendritic cells. It is strongly upregulated in proliferating vascular endothelial cells and some carcinomas. Mature mouse DMC shares 83% and 70% aa sequence identity with rat and human DMC, respectively. Application: Suitable for use in Western blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.01-0.2ug/ml with the appropriate secondary reagents to detect mouse DMC. The detection limit for rmDMC is approximately 2 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 5% cross-reactivity with rhDMC. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice