D8074-90C

DMC (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1)

Size

£825.00

SKU:: D8074-90C
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Ser31-Leu119 from mouse DMC, expressed in E. coli (Q5UW37).
Clone:: 9H112
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Mouse DMC (dendritic cell and monocyte chemokine-like protein; also VCC-1) is a secreted 11kD member of the CXC chemokine family. It is constitutively produced in lung, induced in endothelium by VEGF, and chemoattracts monocytes and dendritic cells. Mouse DMC is 97aa in length. It contains six cysteines with no potential N-linked glycosylation sites. Over aa31-119, mouse MDC shares 83% and 70% aa identity with rat and human MDC, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using mouse lung tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.