DISC1 (Disrupted in Schizophrenia 1, RP4-730B13.1, KIAA0457, SCZD9)

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£594.00

SKU:: D8066-83B
Application:: IHC-Fr
Purity:: Purified by peptide immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide made to an internal region (within residues 700-800) of the mouse DISC1 protein. [Swiss-Prot# Q811T9]. Homology: 100% with mouse and rat proteins and 42% with human protein.
Format:: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
Extra Details:: The 'Disrupted In Schizophrenia' gene locus DISC is associated with patients afflicted with schizophrenia as a result of chromosomal translocations. DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins. DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and NUDEL; proteins which localize receptors to membranes, including alpha-actinin 2 an beta 4-spectrin; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5. Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain. Cellular Localization: DISC-1 localizes to the nucleus, whereas mutant DISC-1 localization occurs mainly in the cytoplasm. It colocalizes with the following organelle structure antibodies: PD1, calnexin, GIANTIN, 58K, Rab4, LAMP-1, RAP1, KDEL, and catalase. Positive Control: Mouse brain sections (neurons and glia) Applications: Suitable for use in Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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