D8065-47D

Diphtheria Toxin

Size

£512.00

SKU:: D8065-47D
Purity:: Purified IgG fraction to a purity of ~95%.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified Diphtheria toxoid.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Diphtheria Toxin catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity. Applications: Suitable for use in ELISA, Fluorescence Microscopy, Western Blot, Immunocytochemistry and conjugation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.