D8065-40

Diphtheria Toxin

Size

£256.00

SKU:: D8065-40
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Diphtheria toxoid.
Clone:: 1.B.234
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Extra Details:: Diphtheria Toxin catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: Optimal dilution to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.