D8065-40-PE

Diphtheria Toxin (PE)

Size

£895.00

SKU:: D8065-40-PE
Application:: FLISA
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Diphtheria toxoid.
Clone:: 1.B.234
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Extra Details:: Diphtheria Toxin catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity. Applications: Suitable for use in FLISA. Other applications have not been tested. Recommended Dilutions: Optimal dilution to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.