D8065-31B
Dipeptidyl Peptidase II (DPPII, Quiescent cell proline dipeptidase, QPP, DPP7)
Size
£761.00
- SKU:
- D8065-31B
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- NS0-derived recombinant human DPPII., Gly22-Leu492.
- Clone:
- 8B15
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Dipeptidyl-peptidase II (DPPII), also known as quiescent cell proline dipeptidase (QPP) and DPP7, shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. They are all prolyl proteases that cleave proteins and peptides after proline residues. However, DPPII is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16. Applications: Suitable for use in ELISA, Neutralization and Western blot. Other applications have not been tested. Recommended Dilutions: Neutralization-preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.12ug/ml (IC50) under conditions in which the enzyme was present at 0.1ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at approximately 1:2 molar ratio of the antibody to the enzyme. Western blot: 1-2ug/ml The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with WesternGloTM Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Optimal dilutions to be determined by the researcher. Hybridoma: mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant human DPPII. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice