D8055

DINE (Damage-induced Neuronal Endopeptidase, ECEL1) (Not for Export EU)

Size

£704.00

SKU:: D8055
Purity:: Serum
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 17-aa peptide sequence, mapping nears the cytoplasmic N-terminus of rat DINE (KLH coupled). No significant sequence homology is seen with NEP, NEPLs or ECEs or other proteins. Species Sequence Homology: mouse (94%) and human (88%).
Format:: Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Extra Details:: DINE (damage-induced neuronal endopeptidase) or Endothelin converting enzyme-like 1 (ECEL1) or X-converting enzyme (Xce) is a 95kD (mouse/rat/human 775aa, chromosome 2q36-q37), type II integral membrane metalloprotease containing a conserved zinc-binding motif and an ENXADX consensus sequence, consistent with gluzinitin. The aa sequence of DINE is 36 and 32 % identical to ECE-1 and NEP, respectively. But, DINE is devoid of enzyme activity like ECE. Unlike NEP, DINE has no proteolytic activity to Ab. However, the enzyme can hydrolyze synthetic NEP substrates and thiorphan, EDTA and phosphoramidon inhibit its activity. DINE also inhibits C2-ceramide induced apoptosis in COS-7 cells. Although, the endogenous substrate for DINE is yet to be identified, its proteolytic activity activates, at least in part, free radical scavenging in damaged neurons. The DINE expression is restricted to brain tissue with predominant expression in hypothalamus, large cholinergic cells in striatum and low expression in virtually all regions of brain except in cerebral cortex, hippocampus and cerebellum. DINE, expression is markedly increased in response to optic, spinal sensory, cortical and thalamic nerve injury. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice