D4016-85A

DNER (Delta and Notch-like Epidermal Growth Factor-related Receptor, Brain EGF Repeat Containing Transmembrane Protein, BET) (Biotin)

Size

£850.00

SKU:: D4016-85A
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Ala26-His637 of mouse DNER expressed in NS0 cell.
Clone:: 9i231
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 50ug BSA per 1ug as a carrier protein. Reconstitute with 200ul sterile PBS. Labeled with Biotin.
Extra Details:: Delta/Notch-like EGF-related Receptor (DNER), also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 8-25ug/ml detects DNER in perfusion fixed frozen mouse brain (cerebellum) sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.