D4016-85
DNER (Delta and Notch-like Epidermal Growth Factor-related Receptor, Brain EGF Repeat Containing Transmembrane Protein, BET)
Size
£856.00
- SKU:
- D4016-85
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Arg29-His637 of human DNER expressed in NS0 cell.
- Format:
- Supplied as a powder lyophilized from a solution containing PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- DNER, also known as brain EGF repeat containing transmembrane protein (BET), is a neuron-specific type I transmembrane notch ligand that is expressed on membranes of dendrites and cell bodies but not axons. It contains ten extracellular EGF-like domains and one follistatin-like domain (between the 9th and 10th EGF-like domains). DNER mediates neuron-glia interactions and is required for the functional and morphological maturation of the cerebellum. The extracellular domains of human and mouse DNER share 89% amino acid sequence homology. Applications: Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry: 5-15ug/ml (used with immersion fixed A172 human glioblastoma cells) Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice