D4015-16
DnaK, CT, aa385-638, Substrate Binding Domain, Recombinant, E. coli
Size
£819.00
- SKU:
- D4015-16
- Molecular Weight:
- 27.7
- Purity:
- ≥ 95% by SDS PAGE; Purified by using conventional chromatography techniques.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 5mM DTT, 10% glycerol.
- Extra Details:
- DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. The protein coding region of the substrate binding domain of DNAK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. Additional amino acid (Met) is attached at N-terminus. Molecular Weight: 27.7kD (255 amino acids) Sequence: MDVKDVLLLD/ VTPLSLGIET/ MGGVMTTLIA/ KNTTIPTKHS/ QVFSTAEDNQ/ SAVTIHVLQG/ ERKRAADNKS/ LGQFNLDGIN/ PAPRGMPQIE/ VTFDIDADGI/ LHVSAKDKNS/ GKEQKITIKA/ SSGLNEDEIQ/ KMVRDAEANA/ EADRKFEELV/ QTRNQGDHLL/ HSTRKQVEEA/ GDKLPADDKT/ AIESALTALE/ TALKGEDKAA/ IEAKMQELAQ/ VSQKLMEIAQ/ QQHAQQQTAG/ ADASANNAKD/ DDVVDAEFEE/ VKDKK Biological Activity: Not determined Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice