D4015-06

DNA, ds (Plasmid)

Size

£1,347.00

SKU:: D4015-06
Purity:: Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. A260nm: 1.0 at 50ug/ml
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0.
Extra Details:: The dsDNA antigen is a 2690bp plasmid purified by alkaline lysis and chromatography. The purification process includes operations to minimize reactivity to antibodies against single-stranded DNA. Use of plasmid DNA in ELISA is an effective method for the detection of anti-double-stranded (ds) DNA antibodies (1). The presence of antibodies to dsDNA is included in the 1982 revised criteria for classification of Systemic Lupus Erythematosus (SLE) (2). Approximately 60-70% of SLE patients have antibodies to dsDNA, and there is considerable evidence to implicate immune complexes containing anti-dsDNA and DNA in the pathogenesis of SLE (3,4). In addition, levels of anti-dsDNA antibodies have been demonstrated to correlate with disease activity in many patients (5,6). Low levels of anti-dsDNA antibodies may occur in other rheumatic diseases (7) and may occur at a very low frequency (<2%) in individuals without any symptoms of rheumatic disease (8). Anti-dsDNA antibodies may appear in rheumatic patients prior to the development of disease manifestations (9). Applications: Suitable for use in standard solid phase immunoassays. Other applications not tested. Purity: Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. Concentration: ~1mg/ml Form: Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Shipping Conditions:: Dry Ice